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mouse kidney marathon readytm cdna  (TaKaRa)


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    TaKaRa mouse kidney marathon readytm cdna
    Figure 1 | Generation of NaDC1-deficient mice. (a) The gene targeting construct containing the Hsv-tk gene and the neomycin resistance gene (phosphoglycerate kinase-neo). Relative positions of all exons are shown in filled boxes. The NotI site used to linearize the construct, the outside probe (filled bar), and the expected sizes of KpnI fragments used for analysis of genomic DNA are indicated. (b) Genotyping the NaDC1 allele by Southern blot analysis. The expected bands of B4.0 kb for the wild-type NaDC1 allele and B6.25 kb for the mutant NaDC1 allele were observed. (c) Northern blot analysis of mRNA (3 mg/lane) from kidney extracts. The blot was analyzed with an NaDC1 <t>cDNA</t> probe containing exons 2–6, and normalized with b-actin. A B2.2-kb band was detected representing the NaDC1 transcript. (d) Reverse transcription-polymerase chain reaction using RNA from kidney and small intestine with NaDC1 specific primers. The reaction was normalized with GAPDH. A B0.76-kb band was detected representing exons 2–6 of the NaDC1 transcript.
    Mouse Kidney Marathon Readytm Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+kidney+marathon+readytm+cdna/Mouse+Liver+Marathon+-Ready+cDNA/pm17410095-99-16-20
    Average 93 stars, based on 78 article reviews
    mouse kidney marathon readytm cdna - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Generation and characterization of sodium-dicarboxylate cotransporter-deficient mice."

    Article Title: Generation and characterization of sodium-dicarboxylate cotransporter-deficient mice.

    Journal: Kidney international

    doi: 10.1038/sj.ki.5002258

    Figure 1 | Generation of NaDC1-deficient mice. (a) The gene targeting construct containing the Hsv-tk gene and the neomycin resistance gene (phosphoglycerate kinase-neo). Relative positions of all exons are shown in filled boxes. The NotI site used to linearize the construct, the outside probe (filled bar), and the expected sizes of KpnI fragments used for analysis of genomic DNA are indicated. (b) Genotyping the NaDC1 allele by Southern blot analysis. The expected bands of B4.0 kb for the wild-type NaDC1 allele and B6.25 kb for the mutant NaDC1 allele were observed. (c) Northern blot analysis of mRNA (3 mg/lane) from kidney extracts. The blot was analyzed with an NaDC1 cDNA probe containing exons 2–6, and normalized with b-actin. A B2.2-kb band was detected representing the NaDC1 transcript. (d) Reverse transcription-polymerase chain reaction using RNA from kidney and small intestine with NaDC1 specific primers. The reaction was normalized with GAPDH. A B0.76-kb band was detected representing exons 2–6 of the NaDC1 transcript.
    Figure Legend Snippet: Figure 1 | Generation of NaDC1-deficient mice. (a) The gene targeting construct containing the Hsv-tk gene and the neomycin resistance gene (phosphoglycerate kinase-neo). Relative positions of all exons are shown in filled boxes. The NotI site used to linearize the construct, the outside probe (filled bar), and the expected sizes of KpnI fragments used for analysis of genomic DNA are indicated. (b) Genotyping the NaDC1 allele by Southern blot analysis. The expected bands of B4.0 kb for the wild-type NaDC1 allele and B6.25 kb for the mutant NaDC1 allele were observed. (c) Northern blot analysis of mRNA (3 mg/lane) from kidney extracts. The blot was analyzed with an NaDC1 cDNA probe containing exons 2–6, and normalized with b-actin. A B2.2-kb band was detected representing the NaDC1 transcript. (d) Reverse transcription-polymerase chain reaction using RNA from kidney and small intestine with NaDC1 specific primers. The reaction was normalized with GAPDH. A B0.76-kb band was detected representing exons 2–6 of the NaDC1 transcript.

    Techniques Used: Construct, Southern Blot, Mutagenesis, Northern Blot, Reverse Transcription, Polymerase Chain Reaction

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Generation and characterization of sodium-dicarboxylate cotransporter-deficient mice.
    Article Snippet: .. Probes for detecting NaDC1, NaDC3, GPX1, SOD2, and SIRT1 transcripts were polymerase chain reaction amplified from mouse kidney Marathon-ReadyTM cDNA (Clontech, Palo Alto, CA, USA) with primers designed from the published Mus musculus NaDC1 mRNA sequence (GenBank accession no. AF201903: forward primer bp no. 121-144; reverse primer bp no. 860-881), Mus musculus NaDC3 mRNA sequence (accession no. BC026803: forward primer bp no.;1135-1157; reverse primer bp no.;21962219), Mus musculus GPX1 mRNA sequence (accession no. NM008160: forward primer bp no. 10-33; reverse primer bp no. 691-714), Mus musculus SOD2 mRNA sequence (accession no. NM013671: forward primer bp no. 44-67; reverse primer bp no. 909-933), and Mus musculus SIRT1 mRNA sequence (accession no. NM019812; forward primer bp no. 470-493; reverse primer bp no. 1553-1575), respectively. ..

    Amplification:

    Article Title: Generation and characterization of sodium-dicarboxylate cotransporter-deficient mice.
    Article Snippet: .. Probes for detecting NaDC1, NaDC3, GPX1, SOD2, and SIRT1 transcripts were polymerase chain reaction amplified from mouse kidney Marathon-ReadyTM cDNA (Clontech, Palo Alto, CA, USA) with primers designed from the published Mus musculus NaDC1 mRNA sequence (GenBank accession no. AF201903: forward primer bp no. 121-144; reverse primer bp no. 860-881), Mus musculus NaDC3 mRNA sequence (accession no. BC026803: forward primer bp no.;1135-1157; reverse primer bp no.;21962219), Mus musculus GPX1 mRNA sequence (accession no. NM008160: forward primer bp no. 10-33; reverse primer bp no. 691-714), Mus musculus SOD2 mRNA sequence (accession no. NM013671: forward primer bp no. 44-67; reverse primer bp no. 909-933), and Mus musculus SIRT1 mRNA sequence (accession no. NM019812; forward primer bp no. 470-493; reverse primer bp no. 1553-1575), respectively. ..

    Sequencing:

    Article Title: Generation and characterization of sodium-dicarboxylate cotransporter-deficient mice.
    Article Snippet: .. Probes for detecting NaDC1, NaDC3, GPX1, SOD2, and SIRT1 transcripts were polymerase chain reaction amplified from mouse kidney Marathon-ReadyTM cDNA (Clontech, Palo Alto, CA, USA) with primers designed from the published Mus musculus NaDC1 mRNA sequence (GenBank accession no. AF201903: forward primer bp no. 121-144; reverse primer bp no. 860-881), Mus musculus NaDC3 mRNA sequence (accession no. BC026803: forward primer bp no.;1135-1157; reverse primer bp no.;21962219), Mus musculus GPX1 mRNA sequence (accession no. NM008160: forward primer bp no. 10-33; reverse primer bp no. 691-714), Mus musculus SOD2 mRNA sequence (accession no. NM013671: forward primer bp no. 44-67; reverse primer bp no. 909-933), and Mus musculus SIRT1 mRNA sequence (accession no. NM019812; forward primer bp no. 470-493; reverse primer bp no. 1553-1575), respectively. ..



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    mouse kidney marathon readytm cdna  (TaKaRa)
    93
    TaKaRa mouse kidney marathon readytm cdna
    Figure 1 | Generation of NaDC1-deficient mice. (a) The gene targeting construct containing the Hsv-tk gene and the neomycin resistance gene (phosphoglycerate kinase-neo). Relative positions of all exons are shown in filled boxes. The NotI site used to linearize the construct, the outside probe (filled bar), and the expected sizes of KpnI fragments used for analysis of genomic DNA are indicated. (b) Genotyping the NaDC1 allele by Southern blot analysis. The expected bands of B4.0 kb for the wild-type NaDC1 allele and B6.25 kb for the mutant NaDC1 allele were observed. (c) Northern blot analysis of mRNA (3 mg/lane) from kidney extracts. The blot was analyzed with an NaDC1 <t>cDNA</t> probe containing exons 2–6, and normalized with b-actin. A B2.2-kb band was detected representing the NaDC1 transcript. (d) Reverse transcription-polymerase chain reaction using RNA from kidney and small intestine with NaDC1 specific primers. The reaction was normalized with GAPDH. A B0.76-kb band was detected representing exons 2–6 of the NaDC1 transcript.
    Mouse Kidney Marathon Readytm Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+kidney+marathon+readytm+cdna/Mouse+Liver+Marathon+-Ready+cDNA/pm17410095-99-16-20
    Average 93 stars, based on 1 article reviews
    mouse kidney marathon readytm cdna - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 1 | Generation of NaDC1-deficient mice. (a) The gene targeting construct containing the Hsv-tk gene and the neomycin resistance gene (phosphoglycerate kinase-neo). Relative positions of all exons are shown in filled boxes. The NotI site used to linearize the construct, the outside probe (filled bar), and the expected sizes of KpnI fragments used for analysis of genomic DNA are indicated. (b) Genotyping the NaDC1 allele by Southern blot analysis. The expected bands of B4.0 kb for the wild-type NaDC1 allele and B6.25 kb for the mutant NaDC1 allele were observed. (c) Northern blot analysis of mRNA (3 mg/lane) from kidney extracts. The blot was analyzed with an NaDC1 cDNA probe containing exons 2–6, and normalized with b-actin. A B2.2-kb band was detected representing the NaDC1 transcript. (d) Reverse transcription-polymerase chain reaction using RNA from kidney and small intestine with NaDC1 specific primers. The reaction was normalized with GAPDH. A B0.76-kb band was detected representing exons 2–6 of the NaDC1 transcript.

    Journal: Kidney international

    Article Title: Generation and characterization of sodium-dicarboxylate cotransporter-deficient mice.

    doi: 10.1038/sj.ki.5002258

    Figure Lengend Snippet: Figure 1 | Generation of NaDC1-deficient mice. (a) The gene targeting construct containing the Hsv-tk gene and the neomycin resistance gene (phosphoglycerate kinase-neo). Relative positions of all exons are shown in filled boxes. The NotI site used to linearize the construct, the outside probe (filled bar), and the expected sizes of KpnI fragments used for analysis of genomic DNA are indicated. (b) Genotyping the NaDC1 allele by Southern blot analysis. The expected bands of B4.0 kb for the wild-type NaDC1 allele and B6.25 kb for the mutant NaDC1 allele were observed. (c) Northern blot analysis of mRNA (3 mg/lane) from kidney extracts. The blot was analyzed with an NaDC1 cDNA probe containing exons 2–6, and normalized with b-actin. A B2.2-kb band was detected representing the NaDC1 transcript. (d) Reverse transcription-polymerase chain reaction using RNA from kidney and small intestine with NaDC1 specific primers. The reaction was normalized with GAPDH. A B0.76-kb band was detected representing exons 2–6 of the NaDC1 transcript.

    Article Snippet: Probes for detecting NaDC1, NaDC3, GPX1, SOD2, and SIRT1 transcripts were polymerase chain reaction amplified from mouse kidney Marathon-ReadyTM cDNA (Clontech, Palo Alto, CA, USA) with primers designed from the published Mus musculus NaDC1 mRNA sequence (GenBank accession no. AF201903: forward primer bp no. 121-144; reverse primer bp no. 860-881), Mus musculus NaDC3 mRNA sequence (accession no. BC026803: forward primer bp no.;1135-1157; reverse primer bp no.;21962219), Mus musculus GPX1 mRNA sequence (accession no. NM008160: forward primer bp no. 10-33; reverse primer bp no. 691-714), Mus musculus SOD2 mRNA sequence (accession no. NM013671: forward primer bp no. 44-67; reverse primer bp no. 909-933), and Mus musculus SIRT1 mRNA sequence (accession no. NM019812; forward primer bp no. 470-493; reverse primer bp no. 1553-1575), respectively.

    Techniques: Construct, Southern Blot, Mutagenesis, Northern Blot, Reverse Transcription, Polymerase Chain Reaction